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Advantages of the MicroSEQ™

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How does the MicroSEQ works?

Genomic DNA is extracted directly from dead or alive bacterial colonies grown under any conditions. The 16S rRNA gene is amplified using universal primers and thermalcyclers. The amplified 16S rRNA gene product is sequenced using dye terminator cycle sequencing chemistry. The sequence reactions are analyzed using automated DNA sequencers and software.

Unknown bacteria samples are identified using the MicroSEQ microbial identification software and 16S rRNA gene sequence database, containing over 1,500 entries. Routine bacterial identification is performed using the first 500 bp of the rDNA. However, there is also the option of using higher-resolution identification based on the full 1,500 bp region (if requested by customer). Data analysis can be done using either automated or manual modes. Outcome predictions is done using the phylogenetic tree tool. The system also has the ability to build user-defined and user-validated custom libraries

The MicroSEQ ID system outperforms phenotypic approach (such as the MALDI-TOF). Click here to read about it in Life Technologies 2012, Genotypic approach to microbial identifications outperforms phenotypic approach, accessed 16 April 2015.

Who will benefit from this system?

Clients who:

  • require identification of Yeast and Mould to species level
  • are investigating environmental contamination
  • have product failures or recalls due to microbial contamination
  • requires cleanroom qualification of technicians
  • requires further identification of medical device microbial flora
  • needs further confirmation/clarification of results from other less accurate ID systems

Contact us now if you are interested to come on-site for a free tour of the facility and inspection of the new ID system. Alternatively, we can also go to you to give a free presentation about the MicroSEQ (depending on where you are located).