Eurofins Australia >> Food Testing >> Honey Authenticity

Honey Authenticity Toolkits by experts

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The most common fraud in honey is adulteration, usually by addition of sugar. However other types of fraud exist, for example the false declaration of botanical or geographical origin. Traceability is therefore crucial for honey and other products from the hive to ensure a fair and sustainable beekeeping sector.

Unique and reliable methods

Eurofins Competence Centres in Nantes, France and Bremen, Germany have developed many analytical techniques to test different kinds of honey. Our analytical toolkits can

  • Detect the addition of exogenous sugars (from whatever source: cane, corn, beet, wheat, rice, etc.).
  • Specific detection of sugar syrups
  • Confirm the floral origin of honey declared on the product's packaging (single-flower honeys)
  • Detect various irregularities such as excessive heat treatment (determination of 5-HMF) or fermentation
  • Quantify the main sugars (glucose, fructose, sucrose)

Why Eurofins are world leaders in honey authenticity testing

The composition and characteristics of honeys are subject to broad variation due to the numerous honey varieties and origins worldwide which makes the detection of possible fraudulent sugar syrup addition am ongoing challenge.  Authenticity testing of honey requires innovation to ensure our techniques stay one step ahead of the fraudsters.

Eurofins has a trusted approach to honey integrity testing with many methods to help perform reliable and decisive risk assessments to protect your brand and honey quality:

  • 1H-NMR profiling of honey
  • C4/C3 Sugars in Honey - Determination of d13C values of protein, honey, fructose, glucose, disaccharides, disaccharides (and oligosaccharides, if present) by EA-IRMS and LC-IRMS for the detection of fraudulent addition of foreign sugars derived from C4 or C3 plants
  • Specific marker rice syrup
  • Colourant 150d
  • Mannose in Honey
  • Determination of beta-fructofuranosidase (foreign invertase) activity in honey (adulteration with énzymatically inverted sugars)
  • Determination of foreign alpha-amylases (different from honey diastase) in honey to detect honey adulteration
  • Honey authenticity by LC-HRMS*

*LC-HRMS – A New Dimension in Honey Authenticity Testing

Research and Development: Eurofins Establishes New Detection Method for Adulteration

Eurofins Honey Competence Centre in Bremen is the first laboratory worldwide to offer the authenticity analysis of honey by liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS) as an ISO 17025 accredited method.

The advantages of LC-HRMS at One Glance 

  • Enables the detection of known (target) and unknown (non-target) honey adulterants in one analytical test.
  • Has a significant higher detection sensitivity for foreign sugar adulterants compared to established honey authenticity testing methods.
  • Is suitable as a multimethod with hundreds of identified marker substances for the simultaneous detection of additions of different types of sugar syrups used for adulteration.
  • Can replace various single methods for certain types of adulteration and thus helps to reduce the analytical effort in terms of analysis costs and testing efficiency.
  • Combined with 1H NMR-Profiling and 13C stable isotope analysis (EA/LC-IRMS), it is the most advanced and reliable testing method for honey authenticity on the market.


Please contact your local Eurofins laboratory for more information about honey testing.