Testing Solutions >> Sterile Products Testing >> Antibiotic Potency Test / Bioassay

Antibiotic Potency Test / Bioassay

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BACKGROUND:

Eurofins | ams is able to perform antibiotic potency testing by the bioassay method. Antibiotic potency is estimated by comparing the inhibition of growth of sensitive micro-organisms produced by known concentrations of the antibiotic being examined against a reference standard substance. The test is performed on raw materials and finished products to confirm the antibiotic activity meets specifications.

 

PRINCIPLE OF TEST:

All test methods are based on the European and British Pharmacopeia: Biological assay of antibiotics, BP Appendix XIV A (Ph. Eur. method 2.7.2)

This is a large-plate format procedure where test and standard preparations are loaded into wells in a randomized latin square arrangement before incubation and reading the zones of inhibition.

List of Antimicrobials available are as shown below.
 

List of Antimicrobials available
Amphotericin B
Bacitracin
Bleomycin
Colistin
Colistimethate
Dihydrostreptomycin
Erythromycin
Framycetin
Gentamicin
Kanamycin
Neomycin
Netilmicin
Nystatin
Polymyxin B
Rifamycin
Spiramycin
Streptomycin
Tobramycin
Tylosin
Vancomycin

 

We can also perform Antimicrobial Activity Tests such as those shown below.
 

List of Antimicrobial Activity Tests
Minimum Inhibition Concentration (MIC) Test
Minimum Bactericidal Concentration (MBC) Test
Minimum Kill Concentration ( MKC) Test
Zone of Inhibition (ZOI) Test